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. Author manuscript; available in PMC: 2010 Nov 15.
Published in final edited form as: J Immunol. 2009 Oct 21;183(10):6667. doi: 10.4049/jimmunol.0900516

Figure 9. Inhibition of egress by SB203580.

Figure 9

(A, B). YFP fluorescence in timelapse images (10 min intervals) of adherent PEM exposed at 0 h to exudate in the presence of either DMSO vehicle (A) or 10 uM SB203580 (B). Egress events that had occurred by 2h are indicated by arrows (lower panels). Scale bar: 20 μm. (C) The proportion of YFP-RH+ cells egressing over 2h is displayed as the mean ± S.E. from either 3 (DMSO) or 8 (SB203580) fields. The data are representative of two similar experiments. (D) Inhibition of egress in ex vivo mixed exudate. Washed donor exudate cells from mice infected for 5d with YFP-RH were labeled in situ with DDAO-SE, mixed (1:9) with unlabeled exudate from wild-type RH-infected mice, and incubated for 1 h in the presence of either vehicle or 10 uM SB203580. Each of the 3 mixes represents a separate pair of donor and host mice. Bars = mean ± S.E. (n = 3). The data are representative of three similar experiments.