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. Author manuscript; available in PMC: 2010 Apr 29.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Dec 31;392(1):36. doi: 10.1016/j.bbrc.2009.12.143

Figure 1.

Figure 1

Yeast cells containing c-myc sclerostin and HA Cyr61 expression plasmids were grown for 3 days at 30°C. Cells were lysed and immunoprecipitation was performed using c-myc (lanes A and D) or HA (lanes B and E) antibodies or no antibodies (lane C). The antibody complexes were precipitated by protein A beads. The precipitates were subjected to SDS-PAGE and separated proteins were transferred to PVDF membranes. The membranes were probed with c-myc or sclerostin (SOST) monoclonal antibodies and chemiluminescence methods.