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. Author manuscript; available in PMC: 2010 Jul 17.
Published in final edited form as: Biochem Biophys Res Commun. 2009 May 9;385(1):49. doi: 10.1016/j.bbrc.2009.05.020

Figure 3. STIM1445-475 peptide interferes with store-dependent and independent CRAC channel activation.

Figure 3

(A-C) Synthetic STIM1445-475 peptide inhibits constitutive ICRAC HEK293 cells were transfected with ORAI1 and CCb9 and ICRAC was recorded in the presence of 20 μM STIM1445-475 peptide or control myelin oligodendrocyte glycoprotein (MOG) peptide in the internal pipette solution. (A) Averages of time-dependent current development (STIM1445-475, n=17; MOG, n=18). (B) Representative I-Vs from the same experiments shown in A extracted at 200 sec. (C) Averages of current values recorded during the time indicated by the black bar in (A). Error bars represent s.e.m. (D-F) Store-dependent activation of native CRAC channel currents in T cells is inhibited by STIM1445-475 peptide. Endogenous ICRAC was activated in Jurkat T cells by passive store depletion with 10 mM BAPTA in the pipette solution in the presence of 20 μM STIM1445-475 or MOG control peptide. (D) Averages of time-dependent current development extracted at -80 mV. (E) Representative I-Vs from the same experiments shown in (D) at 275 sec. (F) Averages of ICRAC values recorded from 280-300 sec (black bar) in experiments shown in (D). STIM1445-475, n=13; MOG, n=13. Error bars represent s.e.m.