Table.
Immunohistochemical Markers Examined, Including Antibody Sources and Staining Parameters
| Marker | Antibody Source | Species | Primary Antibody Dilution | Incubation Temperature | Incubation Time | Pretreatments |
|---|---|---|---|---|---|---|
| Tau | Accurate Chemical & Scientific | Rabbit | 1:100 | 40°C | 30 min | None |
| Neurofilaments | Dako (clone 2F11) | Mouse | 1:100 | 40°C | 30 min | Pepsin (Biomeda) |
| α-Synuclein | Gift of Dr Bernardino Ghetti | Rabbit | 1:400 | 4°C | 24 hr | 90% formic acid |
| α-Synuclein | Zymed | Mouse | 1:100 | 4°C | 24 hr | 90% formic acid |
| GFAP | Dako (clone 6F2) | Mouse | 1:100 | 40°C | 30 min | None |
| GFAP | Gift of Dr Virginia Lee | Rabbit | 1:750 | 40°C | 30 min | None |
| Ubiquitin | Dako | Rabbit | 1:100 | 40°C | 30 min | None |
| Ubiquitin | East Acres Biologicals | Rabbit | 1:500 | 40°C | 30 min | None |
| β-Amyloid | Senetek (4G8) | Mouse | 1:5,000 | 4°C | 24 hr | Pepsin, 90% formic acid |
| Apolipoprotein E | Medix | Goat | 1:250 | 40°C | 30 min | Pepsin, 90% formic acid |
| Apolipoprotein E | Chemicon | Goat | 1:250 | 40°C | 30 min | Pepsin, 90% formic acid |
| Actin | Santa Cruz | Mouse | 1:40 | 4°C | 48 hr | Microwave in 0.01M citrate, pH 6.0, Autozyme (Biomeda) |
| Actin | Sigma | Mouse | 1:100 | 4°C | 48 hr | Microwave in citrate, Autozyme |
| Actin | Sigma | Rabbit | 1:2,000 | 4°C | 48 hr | Microwave in citrate, Autozyme |
| HAM-56 | Cell Marque | Mouse | 1:10 | 40°C | 30 min | Microwave in citrate |
| HLA-DR α-chain | Dako (clone TAL.1B5) | Mouse | 1:100 | 40°C | 30 min | Microwave in citrate |
| CD68 | Dako (clone PG-M1) | Mouse | 1:100 | 40°C | 30 min | Microwave in citrate |
| Amyloid precursor protein | Roche (clone 22C11) | Mouse | 1:200 | 4°C | 24 hr | Microwave in citrate |
| ADF/cofilin | Gift of Dr Hiroshi Abe | Mouse | 1:40 | 4°C | 24 hr | Microwave in citrate |
| ADF/cofilin | Cytoskeleton | Rabbit | 1:125 | 4°C | 24 hr | Microwave in citrate |
| Gelsolin | Sigma | Mouse | 1:500 | 4°C | 24 hr | None |
| MAP1b | Gift of Dr Lester Binder | Mouse | 1:300 | 4°C | 24 hr | Microwave in citrate |
| Tubulin | Gift of Dr Lester Binder | Mouse | 1:500 | 4°C | 24 hr | Microwave in citrate |
| Polyglutamine repeats | Gift of Dr J.-L. Mandel (1C2) | Mouse | 1:500 | 4°C | 24 hr | None |
| Met-enkephalin | INCSTAR | Rabbit | 1:1,000 | 4°C | 24 hr | None |
| NG2 | Chemicon | Rabbit | 1:50 | 4°C | 24 hr | Microwave in citrate |
| Synaptophysin | Biogenics (clone 5Y38) | Mouse | 1:320 | Room temperature | 25 min | 5 min in pressure cooker 15lb/in2 |
GFAP = glial fibrillary acidic protein; ADF = actin depolymerizing factor.