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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Exp Neurol. 2008 Dec 3;216(1):95. doi: 10.1016/j.expneurol.2008.11.015

Figure 4. Addition of OEG enhances the outgrowth of DRG axons on a myelin substrate. Neurons are identified with anti-β-tubulin immunoreactivity after 24 hours in vitro.

Figure 4

A: For the positive control, DRG are grown on laminin and extend elaborate processes.

B: For the negative control, DRG neurons are grown alone on myelin and extend short neurites (short arrows).

C, D: When OEG from wild-type (C) or L1 mutant (D) mice are co-cultured with DRG neurons on myelin, a higher percentage of longer axons are detected than when DRG are grown on myelin alone (B). Scale A–D = 50 μm.

E: The highest percentage of DRG neurons extends processes on laminin and the lowest, on myelin (mean ± SEM). The presence of OEG from either wild-type or L1 mutant mice significantly enhances the percentage of DRG (wild-type and L1 mutant) that extend axons on myelin. The number of neurons sprouting axons on laminin (P < 0.0001) and OEG (P < 0.0001) substrates are significantly different from those grown alone on a myelin substrate. All are significantly different from laminin (P < 0.02). *Significantly different from myelin (P < 0.0001).

F: The average maximal axon length was shortest on myelin alone and longest on laminin. Regardless of genotype, OEG promoted significantly longer DRG axons than when grown on myelin alone. DRG processes grown on laminin (mean ± SEM; P < 0.0001) and on myelin with OEG (P < 0.02) are significantly longer than those grown on myelin alone. All are significantly different from laminin (P < 0.004). *Significantly different from myelin (P < 0.02).