Figure 1.

TDP43-EGFP is localized to the nucleus when expressed in primary cortical neurons. A, Schematic of TDP43-EGFP showing the RNA-recognition motifs (RRMs) 1 and 2, as well as the glycine-rich domain (GRD). EGFP is fused to the C terminus of the protein. The red line denotes the approximate location of the A315T mutation. B–G, Neurons expressing TDP43(WT)-EGFP (B–D) or TDP43(A315T)-EGFP (E–G) demonstrate nuclear localization of the protein when imaged by fluorescence microscopy. B, E, EGFP fluorescence. C, F, mCherry fluorescence. D, G, Merged images with EGFP fluorescence in green, mCherry in red, and overlap in yellow. Scale bar, 10 μm.