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. 2010 Feb 8;3:1. doi: 10.3389/neuro.16.001.2010

Figure 7.

Figure 7

Quantitative NSCs differentiation assay. Data are expressed as percentages of cells positive for GFAP, β-Tubulin, GALC/O4 and Nestin markers over the total number of cells counted in the same fields (see Materials and Methods for details). NSCs have been differentiated for 7 days in vitro. By increasing the length of the Gly-spacer, the percentage of Nestin+ significantly decreased. Despite that GFAP+ cell fraction increased inversely. β-Tubulin+ and GalC/O4+ cell percentages did not significantly differ among the tested substrates and assembled SAPs. NSCs cultured over plastic well surfaces (Negative Control), being composed of clusters of mainly Nestin+ cells, were not included in this graph. Values are expressed as means ± standard error of the mean. * = significantly different values (P < 0.05).

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