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. Author manuscript; available in PMC: 2010 Feb 12.
Published in final edited form as: Clin Cancer Res. 2005 May 15;11(10):3743. doi: 10.1158/1078-0432.CCR-04-1990

Fig. 1.

Fig. 1

The activity of AdTSTA-FL and the induction with androgen in a panel of prostate cancer cell lines. A, schematic representation of the AdTSTA-FL. The two TSTA components of activator (GAL4-VP16 driven by PSE-BC) and reporter (Fluc driven by 5xGAL4) are inserted into E1 region of recombinant adenovirus. B, AdTSTA-FL activity on a panel of prostate cancer cell lines. The cell lysates were harvested 2 days after infection and subjected to FL activity and Western analysis with anti-AR antibody. Fold inductions calculated by the activity ratio with 10 nmol/L R1881 over 10 μmol/L casodex are indicated at the top in parentheses. The activity difference between the AR-positive cell lines and the AR-negative lines (DU-145 and PC-3) is statistically significant (P < 0.01). C, AdTSTA-FL activity in MDA PCa 2b. The cells were infected and incubated with 10 μmol/L casodex, 10 nmol/L R1881, and 5 and 50 nmol/L hydrocortisone.