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. Author manuscript; available in PMC: 2010 Feb 12.
Published in final edited form as: Trends Mol Med. 2003 Oct;9(10):421. doi: 10.1016/j.molmed.2003.08.005

Figure 3.

Figure 3

Estimation of the sensitivity of optical imaging. Prostate cancer cells, LNCaP, were infected by recombinant adenovirus expressing FL driven by CMV promoter (AdCMV-FL) at MOI 10. Two days after infection, cells were harvested and resuspended in 50 μl sterile phosphate-buffered saline + 50 μl Matrigel (BD Biosciences), and injected intra-peritoneally. On the same day of cell implantation, mice were imaged in Xenogen IVIS charged coupled device camera after administration of 200 μl D-luciferin substrate (15 mg/ml). Optical signal intensity (listed below images in relative luminescent units per min of acquisition, RLU/min) is roughly proportional to the number of AdCMV-FL-infected cells (listed above the images). The negative control cells were infected with AdCMV-TK.