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. 2010 Feb 15;5(2):e9203. doi: 10.1371/journal.pone.0009203

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Soubry et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Monoclonal antibodies were used to detect the expression of Kaiso (anti-mouse 6F) and alpha-tubulin (anti-rat YOL1/34) in SK-LMS-1 cells. An Alexa 488-conjugated anti-mouse secondary antibody was used in case of Kaiso detection, and an Alexa 594-conjugated anti-rat secondary antibody for alpha-tubulin. DNA was stained with DAPI. Cells were imaged (100× objective lens) with a Zeiss Axiophot microscope while focusing on the cytoplasmic microtubular network.