Figure 5. Kaiso associates with PCM proteins in a molecular complex.
Lysates were prepared from HeLa-GFP-centrin cells. Immunoprecipitates (IP), using either anti-Kaiso (pAb S1337) or irrelevant antibodies, were separated by SDS-PAGE, followed by western blotting using anti-Kaiso (pAb S1337), anti-pericentrin (pAb ab4448) and anti-γ-tubulin antibodies. Lysate samples are loaded in the left lanes. No specific protein bands were observed in the IP with irrelevant antibody or in the preclear bead eluates. Results are representative of four experiments. It is unclear why the pericentrin and γ-tubulin bands present as doublets. The pericentrin gene is known to encode numerous alternative splice forms [68].