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. 2010 Feb 15;5(2):e9203. doi: 10.1371/journal.pone.0009203

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Soubry et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Column A shows pictures made after focusing on the midbody of SK-LMS-1 cells: Kaiso is present at the midbody (long arrow) while p120ctn is located at the cleavage furrow (double short arrow) between the two forming cells. Column B shows pictures of the same cells while focusing on the protrusions at the cell borders. Kaiso and p120ctn partly colocalize there. Pictures in column C are magnifications of the free edge of one of the daughter cells. Cells were stained with a polyclonal antibody (S1337) for Kaiso detection, and with a monoclonal antibody (pp120) for p120ctn detection, followed by an Alexa 488-conjugated anti-rabbit secondary antibody for Kaiso, and an Alexa 594-conjugated anti-mouse secondary antibody for p120ctn. DNA was stained with DAPI. Cells were imaged (63× objective lens) with a Zeiss Axiophot microscope.