Table 2. Overview of CD34+ cell selections and NK cell culturing procedures.

			Method I - H3000		Method I - Stemline I		Method I - Stemline II
	CD34 (%)	CD34 cells (×106)	CD56 (%)	CD56 cells (×109)	CD56 (%)	CD56 cells (×109)	CD56 (%)	CD56 cells (×109)
UCB1	78	1.10	50	0.57	74	1.20	87	2.20
UCB2	55	0.32	88	0.68	65	0.15	82	0.15
UCB3	93	0.52	94	3.10	43	0.55	71	0.50
UCB4	69	0.45	n.d.	n.d.	99	3.50	57	0.12
UCB5	87	0.20	n.d.	n.d.	75	0.40	48	0.02
UCB6	83	0.94	n.d.	n.d.	97	10.0	49	0.12
mean	78	0.59	77	1.50	75	2.70	66	0.51
SD	14	0.36	24	0.14	21	4.00	17	0.82
range	55–93	0.20–1.10	50–94	0.57–3.10	43–99	0.15–10.0	48–87	0.02–2.20
median	81	0.49	88	0.68	75	0.90	64	0.14

Table 2 shows a summary of all different experiments and the corresponding UCB donors using the different culture methods I–III and media (see Figure 1). The purity and the total number of CD34⁺ cells after immunomagnetic selection determined by FCM are depicted for each UCB donor. The CD56 content after 5 weeks (Method I and II) or 6 weeks (Method III) of culture was analyzed by FCM as described in Materials and Methods. Numbers of NK cells represent a theoretical calculation based on the actual expansion rates of CD56⁺ NK cells and were calculated using the initial numbers of CD34⁺ cells from each UCB multiplied by the NK cell expansion rate from each culture procedure. The mean ± standard deviation (SD), range and median are calculated for each method and medium separately.