Figure 3. Expression and signaling in mTOR pathway in response to stress stimuli and rapamycin.

(A) Western blot analysis of extracts from matched-control and SIRT1 depleted HeLa cells after stress treatments as indicated. (B) SIRT1-depleted and matched-control HeLa cells, and (C) Wild-type, SIRT1 null and TSC2 null MEFs were either mock-treated (vehicle alone) or treated with 25nM rapamycin for 1 hr followed by Western blot analysis for mTOR activity. S+: SIRT1+/+, S-: SIRT1−/−, T+: TSC2+/+, T-: TSC2−/−.