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. 2010 Feb 15;5(2):e9222. doi: 10.1371/journal.pone.0009222

Figure 1. Purification and oligomeric status of Mtb-BirA.

Figure 1

(a) Analysis of the purified recombinant Mtb-BirA by SDS-PAGE on a 12.5% gel. Molecular-mass protein standards are also indicated. (b) Gel-filtration chromatography profile of the affinity purified Mtb-BirA for subunit determination. Calibration curve for the packed Sephadex G-200 column (2.5 cm×90 cm) obtained by plotting the logarithms of the molecular weight (log MW) of the standards (x axis) versus the elution volumes (y axis) is shown as an inset.