Table 1. A comparative analysis of Mtb-BirA and other known bacterial BirA structures.

Organism (Function)	PDB	Identity (%)	Naligned	RMSD (Å)‡	Space group	VM	Solvent Content (%)	Mol per a.u.	Oligomeric state in solution	Ligand	Reference
M. tuberculosis (M)	3l2z	100	238	0.00	P212121	2.2	44	2	monomer	-	This study
M. tuberculosis	3l1a	100	238	0.82	P212121	1.7	28	2	monomer	-	This study
M. tuberculosis	2cgh	100	238	0.46	P212121	2.3	45.4	2	monomer	-	Unpublished
E. coli (B)	1bia	30.2	215	1.80	P43212	2.8	55.6	1	monomer	-	[10]
E. coli	1bib	30.4	217	1.76	P43212	2.8	55.5	1	dimer†	Biotin	[10]
E. coli	1hxd	30.1	219	1.71	C2221	2.9	57.5	2	dimer†	Biotin	[10]
E. coli	2ewn	29.5	220	1.72	P43212	3.8	67.8	2	dimer	Biotinol-5′-AMP	[12]
P. horikoshii (M)	1wq7	27.2	206	1.55	P21	2.1	38.1	2	dimer	-	[11]
P. horikoshii	1wpy	26.7	209	1.50	P21	2.1	42.5	2	dimer	Biotin	[11]
P. horikoshii	2fyk	26.7	209	1.49	P21	2.1	42.5	2	dimer	Biotin & ADP	Unpublished
P. horikoshii	2dto	26.7	209	1.52	P21	2.1	42.5	2	dimer	Biotin & ATP	Unpublished
P. horikoshii	1wqw	26.7	209	1.53	P21	2.1	42.5	2	dimer	Biotinyl- 5′-AMP	[11]
P. horikoshii	2 ejg	28.3	198	1.54	P21	2.4	49.2	2	dimer	BCCP	[13]
A. aeolicus (M)	3fjp	21.4	196	1.52	P21	2.4	48.2	2	monomer	-	[14]
A. aeolicus	3efs	21.4	196	1.52	P212121	2.3	45.5	2	monomer	Biotin & ATP	[14]
M. jannaschii (M)	2ej9	23.8	238	1.70	C2	2.8	56.1	1	monomer	Biotin	Unpublished

M: Monofunctional biotinylation activity; B: Bifunctional biotinylation and repressor activity; ^‡The Cα superposition was performed between chain A of hMtb-BirA (3l2z) and chain A of other PDBs; V_M: Mathews Coefficient; ^†Weak dimer.