Fig. 6.
Capybara basilar artery perivascular nerves labelled (black precipitate) for ETA (A) and ETB (B) receptors. In a and b note axons (Ax) and Schwann cell profiles (Sch) displaying immunoreactivity (arrows) for ETA and ETB receptors, respectively; some labelling is seen in the granular vesicles. In C note ETB localisation in the Schwann cells only, while associated axon varicosities is negative for ETB receptors. Bars: 0.5 µm. Rabbit polyclonal antibodies ETA and ETB (Alomone Labs, Jerusalem, Israel) were used at 1:400 in pre-embedding ExtrAvidin method. The ETA receptor antibody (AER-001) recognises intracellular (C-terminus) epitope corresponding to amino acid residues 413-426 of rat ETA peptide (Accession P26684), while the ETB receptor antibody (AER-002) recognises intracellular (i3 loop) epitope corresponding to residues 298-314 of rat ETB peptide (Accession P21451). Both antibodies were affinity purified on immobilized antigens. (A-C are modified from Loesch et al., J Mol Histol 2005; 36: 25-34 [Kluwer and Springer Science and Business Media], which is kindly acknowledged)