Fig. 7.

Endothelin and its receptors on human sural nerve. a Location of human sural nerve: (From Aktan Ikiz et al. 2005) sn:sural nerve, lm:lateral malleolus, idcn: intermediate dorsal cutaneous nerve. b Distribution of ET-1, ET_A and ET_B receptors on human sural nerve sections revealed by in vitro autoradiography using radiation-sensitive film (low resolution). c Microscopic localisation of ET-1 on a section of human sural nerve. Left panel is a dark-field illumination, ‘high resolution’ autoradiograph of a section incubated in ¹²⁵I-labelled ET-1 and detected using nuclear emulsion where white grains show receptor binding sites. Right panel is the haemotoxylin and eosin stained tissue. There is strong binding to the epineurial vessels (BVS), perineurium (arrowheads) and vasa nervorum (small cell clusters within the nerve fascicles surrounded by the perineurium). Scale bars = 2 mm for autoradiographs in B and 50 µm for C (B and C modified from Dashwood and Thomas 1997)