Fig. 1.

CCN2 promoter is expressed in myofibroblasts in response to bleomycin. Skin of mice treated with PBS or bleomycin was fixed, sectioned, and stained with DAPI to detect nuclei, anti-α-SMA antibody to detect myofibroblasts and anti-CCN2 promoter antibody (10× magnification of dermal tissue). The percentage of fibroblasts within the wound that were α−SMA positive, CCN2- positive, and CCN2/α-SMA positive was calculated as described in Methods. Representative data from n = 4 wounds from 4 separate animals are shown