Wet weight (A), dry weight (B), percent water (C), and DNA content (D, E), of the annuli (open) and cores (closed) of free-swelling (boxes) and dynamically loaded (triangles) constructs over the 6-week culture period (n=4-9). Significant effect of time was observed for these samples (ANOVA p<0.05). * represents significant differences versus day 0 constructs (ANOVA p<0.05; post hoc p<0.05); † represents significant differences versus free-swelling controls (ANOVA p<0.01; post hoc p<0.05); § represents significant differences versus the respective cores (ANOVA p<0.001; post hoc p<0.05).