Figure 19.1.

(A) Substrate-induced cleavage of the SCOT protein. Lanes 1 and 2 contain the purified SCOT protein incubated in the absence or presence of acetoacetyl-CoA, respectively. The protein bands, namely full-length SCOTand the fragments generated after fragmentation, are shown in a Coomassie-stained gel. (B) An immunoblot using the anti-SCOT serum showing positive reaction with the amino-terminal fragment after cleavage of the protein. (C) An immunoblot with the anti-3NT antibody showing positive cross-reaction with the carboxy-terminal fragment only. The positions of the SCOT full-length protein (58 kDa) and carboxy (COOH, 21 kDa)- and amino (NH₂, 37 kDa)-terminal fragments are indicated by arrows.