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. Author manuscript; available in PMC: 2010 Feb 13.
Published in final edited form as: Comp Immunol Microbiol Infect Dis. 2008 Aug 28;32(6):477. doi: 10.1016/j.cimid.2008.06.001

Fig. 1.

Fig. 1

Expression of TIRAP and MyD88 dominant-negative (D/N) constructs in bovine aortic (BAEC) and pulmonary artery (BPAEC) endothelial cells. BAEC (lanes 1, 3 and 5) and BPAEC (lanes 2, 4 and 6) were stably transfected with either vector alone (lanes 1 and 2), or vector containing cDNA encoding either the TIR domain of MyD88 (lanes 3 and 4) or full-length TIRAP containing P136 mutated to H136 (lanes 5 and 6). An anti-FLAG antibody was used to detect the expression of the FLAG-tagged constructs. Molecular mass (in kDa) is indicated.