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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Pediatr Pulmonol. 2009 Oct;44(10):1003. doi: 10.1002/ppul.21092

Fig. 4. CF-correctors can increase the amount and function of S1118F-CFTR.

Fig. 4

(A) HEK-293 cells were transiently transfected with WT- or mutant CFTR cDNA, treated for 16 hours at 37°C with 5 µM CF correctors C1 or C3, lysed, immunoprecipitated and probed for CFTR as before. The experiments were repeated for 4 times. (B) Iodide efflux assay in HEK-293 cells transiently transfected with S1118F-CFTR with or without CF-corrector C3 treatment (5 µM). PKA activating agonist (cocktail containing 10µM forskolin, 100µM IBMX and 200µM cpt-cAMP) was used to activate CFTR. Line graph is Iodide efflux (nmol/min) as mean of 4 experiments with standard error mean (SEM). (C) Bar graph is maximal Iodide efflux at 2 min after adding the agonist cocktail as mean of 4 experiments with SEM (calculated by subtracting efflux data at 1 min from that at 2 min).