Skip to main content
. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Am J Trop Med Hyg. 2009 Mar;80(3):475.

FIGURE 1.

FIGURE 1

Flow cytometry analysis of whole blood cells gated on CD3+/CD4 + cells within the lymphocyte gate, stained with anti-CD25 and used to define CD3+/CD4+/CD25low, CD3+CD4+CD25medium, and CD3 + CD4 + CD25high cells. Staining gates for CD3, CD4, and CD25 were determined using thresholds based on the fluorescence minus one (FMO) “no stain” controls. After examination of the scatter plots of all patients, a threshold was set between CD25medium and CD25high cells (line between the blocks denoting Tact and Treg, respectively). These thresholds, and those provided by the FMO controls, were then used throughout for all analyses.