Fig. 8.
Regulation of proteasome activity by PTEN. PTEN-expressing LN229 and PTEN-deficient U87MG cell lines were incubated for 48 h in serum-free MCDB 104 medium. Cells were washed with PBS and lysed and the CT-like proteasome activity was assayed in 10 μg of protein extracts. Gel shows an illustrative Western blot of PTEN protein levels (n=3). * p < 0.05 compared to U87MG cells.