MMP-9 overexpression results in altered levels of NICD, β-catenin, COX-2 and i-NOS. Caco2-BBE cells stably transfected with a pEGFP plasmid, with or without the MMP-9 gene were plated on 6 well filters at confluency and cell lysates were collected and immunoblotted for 6A(i): MMP-9, 6A(ii): NICD, 6A(iii): β-catenin, 6B(i): COX-2 and 6B(ii): i-NOS. β-tubulin was used as the loading control. Western blots were quantitated by scanning densitometry and graphed adjacently. Each lane shows protein (30μg/lane). Values are representative of three experiments, mean ± SE; *p<0.05.