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. 2009 Nov 11;29(45):14211–14222. doi: 10.1523/JNEUROSCI.3948-09.2009

Figure 1.

Figure 1.

Expression of Robo-1 in vomeronasal sensory neurons. A–H, In situ hybridization of coronal sections of vomeronasal organ isolated from E16 (A, E), E18 (B, F), P0 (C, G), and P15 mice (D, H) with DIG labeled cRNA probes specific for robo-1 (A–D) or robo-3 (E–H). From E16 to P15, low levels of robo-1 transcripts are detected in vomeronasal sensory neurons. In contrast, robo-3 is not detected in vomeronasal sensory neurons at these developmental stages. Scale bars, 250 μm. I–N, Sagittal sections of the accessory olfactory bulb from P0 (I, J) and adult (K–N) mice stained with Robo-1 antibodies (I–L), EC lectin (M, N), and Hoechst (I–N). Robo-1 protein is observed on vomeronasal axons in the nerve layer (arrowhead) and in the mitral cell layer (arrow) of the accessory olfactory bulb (I, J) at P0. Robo-1 is also detected in the nerve and glomerular layers of the accessory olfactory bulb in adult wild-type mice but not in robo-1−/−mice (K, L). Dotted lines outline the nerve and glomerular layers of the AOB in K–N. Scale bars: 125 μm (I, K–N); 50 μm (J).