Figure 2.
Adhesion of PMN to tumour cells. 2 × 106 DiI-labeled PMN were added to culture wells containing confluent dermal fibroblasts (fibroblasts), HMVEC (HMVEC), HT-29 (HT-29), HT-29 with 10 μg/mL of mouse anti-human CD44 monoclonal antibody, 10 μg/mL of mouse anti-human CD162 monoclonal antibody and 10 μg/mL of mouse anti-human CD18 monoclonal antibody in the medium (HT-29 + AbCocktail), FaDu (FaDu), FaDu with the Ab Cocktail (FaDu + AbCocktail), and HMVEC previously treated with 10 U/mL of human recombinant IL-1β for 5 hours (HMVEC + IL-1B) and incubated at 37°C for 30 minutes. After washing, adhered PMN were evaluated as described in the Methods Section. Bars represent the mean ± SEM of 7 independent experiments performed by sixtoplicate. Statistical significance was assessed using ANOVA test; *P < .05, **P < .01, and ***P < .001 when compared with HMVEC group.