Figure 3.

Tumour cell-induced PMN transmigration through human microvascular endothelium. DiI-labeled PMN were added to 3 μm pore size cell culture inserts previously coated with HMVEC cultured at confluence. Inserts were placed upon culture wells containing confluent human dermal fibroblast (fibroblasts), HT-29 (HT-29), FaDu (FaDu) and HMVEC previously treated with 10 U/mL of human recombinant IL-1β for 5 hours (HMVEC + IL-1B). See Methods. After 3 hours at 37°C, PMN in the lower side of the system were evaluated as described in the Methods section. Bars represent the mean±SEM of 7 independent experiments performed by sixtoplicate. Statistical significance was assessed using ANOVA test; *P < .05, and ***P < .001 when compared with fibroblasts group.