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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: J Immunol. 2010 Jan 18;184(4):2065. doi: 10.4049/jimmunol.0902386

Figure 1. Spleen and inner inguinal lymph node cells from C57BL/6 mice receiving UPEC infection secrete IL-17A.

Figure 1

(A) Mice were sensitized transurethrally with either PBS (open symbols) or UPEC (filled symbols) on days 0, 14, and 28, and were sacrificed on day 33. Upon sacrifice, splenocytes from individual animals (n=10) were stimulated with medium alone, 5 µg/ml α-CD3 mAb, or 25 µg/ml whole cell lysate from UPEC strain CFT073. Each symbol represents an individual animal while bars represent the median values. (B) Mice were sensitized transurethrally with either PBS (open bars) or UPEC (filled bars) on days 0 and 7 and sacrificed 2 days after the second sensitization, day 9. Pooled inguinal lymph node cells from each group of animals (n=6) were plated in replicate wells and stimulated as in (A). Bars and error represent the mean ± SEM of triplicate ELISA wells in a representative experiment. n.d., not detectable.