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. Author manuscript; available in PMC: 2011 Feb 2.
Published in final edited form as: Mol Cancer Ther. 2010 Feb 2;9(2):336. doi: 10.1158/1535-7163.MCT-09-0589

Figure 2. Dysregulated Keap1-Nrf2 pathway in prostate cancer cells.

Figure 2

(A) Real-time RT-PCR analysis of Keap1 and Nrf2 expression among prostate cancer cells. The expression levels in PC3 cells are arbitrarily valued as 1. Columns, mean (n = 3); bars, standard deviation. (B) Western blot analysis of Keap1-Nrf2 in prostate cancer cells. Immunoblot with nuclear protein showing increased Nrf2 levels in DU-145 cells and other prostate cancer cells. To detect Keap1 expression, equal amount of whole cell extract was loaded. GADPH, and lamin B were used as normalization controls (C) Real-time RT-PCR analysis of Nrf2 target genes, HO1, NQO1, Gclc, and GSR in prostate cancer cells. The expression levels in PC3 are arbitrarily valued as 1. Columns, mean (n = 3); bars, standard deviation.