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. Author manuscript; available in PMC: 2010 Feb 15.
Published in final edited form as: Mol Cancer Ther. 2007 Sep;6(9):2572. doi: 10.1158/1535-7163.MCT-07-0172

Figure 5.

Figure 5

Effects of MSA on the recruitment of Sp1 or Sp1-like proteins and Sp3 protein to the survivin promoter. The effects of MSA in vivo on binding of Sp1 or Sp1-like proteins and Sp3 protein to the survivin promoter were examined by the chromatin immunoprecipitation assay. C4-2 cells were treated in the presence (+) or absence (−) of 5 μmol/L MSA and immunoprecipitated with antibodies against Sp1, Sp3, or IgG. Coprecipitated DNA was amplified by PCR using primers that flank the regions between −161 and −124 bp in the survivin promoter. The presence of total survivin promoter DNA in the soluble chromatin before immunoprecipitation was included as input.