Table 1. Primer and probe sequences.
Primer/Probe # | Gene or TC Identifier | Direction | Sequence 5′→3′ |
1054 | TC7799tph-1 | F | AAGGACGGCAAGTAGTAAGGA |
1059 | TC7799tph-1 | R | AACAATTCATTTCTTGTAGC |
1248 | TC7799tph-1 | P | VIC-ATCGGTGAGCGTATGGCCGAAGG- TAMRA |
1251 | TC7799tph-1 | R | CTACTACAGCTACTTGTCCCTCACCTT |
1252 | TC7799tph-1 | F | GACCGATTTAAACAGTTGCAGTTC |
1765 | TC7872 | F | GGCCTATGTTGTACATGTCAACGT |
1766 | TC7872 | R | ATATCACCAATAACACCATCGATACC |
1767 | TC7872 | P | 6FAM-ACCGGGACCTGATGGCGTGGAC-IB |
1771 | TC8016 | F | GACGACCGGGACCTGTTG |
1772 | TC8016 | R | TGGTGGACAATGGTCACAACTT |
1773 | TC8016 | P | 6FAM-CTGGAGAACCTGGTGCAC-IB |
1774 | TC8065 | F | TGAAAGCCTTGTTTTACGAGCTAA |
1775 | TC8065 | R | ACCTTTTGGACATCGGTCAGA |
1776 | TC8065 | P | 6FAM-TTTCCAGCTCATTGCCAATGTAG-IB |
1778 | TC8225 | R | GCATGGACAGTAATGAGCATCTG |
1779 | TC8225 | P | 6FAM- AATGGCAAAACTGGAACGCCAGGTAGCA- IB |
1787 | TC7803 | F | GAAATCCCGGAAGAGCCG |
1789 | TC7803 | P | 6FAM-AGGAAATAGTGGCAGTGCT-IB |
1795 | TC7967 | P | 6FAM-AACACGACGTTAAGATTGAT-IB |
1796 | TC8225 | F | GGTGAACAGGGACCACTTGGTAG |
1846 | TC7920 | F | AGGTACTGCTGGAACACCTGGC |
1848 | TC7920 | P | 6FAM-ACCCGGAGAGCCTGGA-MGBNFQ |
1852 | TC7803 | R | CCGTCAGCTCCTGGCTGT |
1853 | TC7967 | R | AATGATGATGAAAACCAATGCACA |
1887 | TC7967 | F | ACCTGGACAACCAGGAGAACG |
1889 | TC7920 | R | GCTGCATCACCGCCTGGTAT |
1899 | Wb cut-1.0 | P | 6FAM-CGAAGACAATCAAGCTCTA- MGBNFQ |
1900 | Wb cut-1.0 | F | CGTCGACGTGAGGACTGATATC |
1901 | Wb cut-1.0 | R | CATTGGTTGTCCACCGAGG |
1908 | Wb cut-1.1 | F | GTCCACTCCTGCTTTGTGGAC |
1909 | Wb cut-1.1 | R | CCCATAAACATTGAGAAGCCG |
1910 | Wb cut-1.1 | P | 6FAM- ACGGTGGAAATTCTGAATGCAGTACAAAA CG-TAMRA |
1911 | Wb cut-1.2 | F | CACACAAATTGAAGTTTCCGAAATT |
1912 | Wb cut-1.2 | R | TTATGATAAACCGGTTGTCCAATG |
1913 | Wb cut-1.2 | P | 6FAM- TGCCTGTATGTCGATATGAGATTCTTGAT GGTG-TAMRA |
1936 | TC7859Wb-col-2 | F | 6FAM-CAACCTCATATTGAATGGTGTT- MGBNFQ |
1938 | Wb-cut-1.2 | F | AAATGAAGAGTTTACCTCAT |
1939 | Wb-cut-1.2 | R | CCGGTTATTGACATACATA |
*Vasuki, et al. (ref # 26).
Gene Identifier = TIGR Cluster Number (TC) available at http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/gimain.pl?gudb=b_malayi.
F = Forward, R = Reverse, P = Probe (Forward orientation).
6FAM & VIC = Fluorescent Molecule on 5′ end of probe, TAMRA = Fluorescent Quencher (Applied Biosystems, Inc.), MGBNFQ = Minor Groove Binding Non-Fluorescent Quencher (Applied Biosystems, Inc.), IB = Iowa Black (Integrated DNA Technologies, Inc., Coralville, IA, USA).