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. 2010 Feb 16;4(2):e602. doi: 10.1371/journal.pntd.0000602

Table 1. Primer and probe sequences.

Primer/Probe # Gene or TC Identifier Direction Sequence 5′→3′
1054 TC7799tph-1 F AAGGACGGCAAGTAGTAAGGA
1059 TC7799tph-1 R AACAATTCATTTCTTGTAGC
1248 TC7799tph-1 P VIC-ATCGGTGAGCGTATGGCCGAAGG- TAMRA
1251 TC7799tph-1 R CTACTACAGCTACTTGTCCCTCACCTT
1252 TC7799tph-1 F GACCGATTTAAACAGTTGCAGTTC
1765 TC7872 F GGCCTATGTTGTACATGTCAACGT
1766 TC7872 R ATATCACCAATAACACCATCGATACC
1767 TC7872 P 6FAM-ACCGGGACCTGATGGCGTGGAC-IB
1771 TC8016 F GACGACCGGGACCTGTTG
1772 TC8016 R TGGTGGACAATGGTCACAACTT
1773 TC8016 P 6FAM-CTGGAGAACCTGGTGCAC-IB
1774 TC8065 F TGAAAGCCTTGTTTTACGAGCTAA
1775 TC8065 R ACCTTTTGGACATCGGTCAGA
1776 TC8065 P 6FAM-TTTCCAGCTCATTGCCAATGTAG-IB
1778 TC8225 R GCATGGACAGTAATGAGCATCTG
1779 TC8225 P 6FAM- AATGGCAAAACTGGAACGCCAGGTAGCA- IB
1787 TC7803 F GAAATCCCGGAAGAGCCG
1789 TC7803 P 6FAM-AGGAAATAGTGGCAGTGCT-IB
1795 TC7967 P 6FAM-AACACGACGTTAAGATTGAT-IB
1796 TC8225 F GGTGAACAGGGACCACTTGGTAG
1846 TC7920 F AGGTACTGCTGGAACACCTGGC
1848 TC7920 P 6FAM-ACCCGGAGAGCCTGGA-MGBNFQ
1852 TC7803 R CCGTCAGCTCCTGGCTGT
1853 TC7967 R AATGATGATGAAAACCAATGCACA
1887 TC7967 F ACCTGGACAACCAGGAGAACG
1889 TC7920 R GCTGCATCACCGCCTGGTAT
1899 Wb cut-1.0 P 6FAM-CGAAGACAATCAAGCTCTA- MGBNFQ
1900 Wb cut-1.0 F CGTCGACGTGAGGACTGATATC
1901 Wb cut-1.0 R CATTGGTTGTCCACCGAGG
1908 Wb cut-1.1 F GTCCACTCCTGCTTTGTGGAC
1909 Wb cut-1.1 R CCCATAAACATTGAGAAGCCG
1910 Wb cut-1.1 P 6FAM- ACGGTGGAAATTCTGAATGCAGTACAAAA CG-TAMRA
1911 Wb cut-1.2 F CACACAAATTGAAGTTTCCGAAATT
1912 Wb cut-1.2 R TTATGATAAACCGGTTGTCCAATG
1913 Wb cut-1.2 P 6FAM- TGCCTGTATGTCGATATGAGATTCTTGAT GGTG-TAMRA
1936 TC7859Wb-col-2 F 6FAM-CAACCTCATATTGAATGGTGTT- MGBNFQ
1938 Wb-cut-1.2 F AAATGAAGAGTTTACCTCAT
1939 Wb-cut-1.2 R CCGGTTATTGACATACATA

*Vasuki, et al. (ref # 26).

Gene Identifier = TIGR Cluster Number (TC) available at http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/gimain.pl?gudb=b_malayi.

F = Forward, R = Reverse, P = Probe (Forward orientation).

6FAM & VIC = Fluorescent Molecule on 5′ end of probe, TAMRA = Fluorescent Quencher (Applied Biosystems, Inc.), MGBNFQ = Minor Groove Binding Non-Fluorescent Quencher (Applied Biosystems, Inc.), IB = Iowa Black (Integrated DNA Technologies, Inc., Coralville, IA, USA).