Table 2. Detergent-resistance percentage (conversion rate) of E. histolytica trophozoites treated with treatment solution at different times.

Treatment Solution a	Incubation Time (h)	Viability (FDA) (% ± S.D.) b	Conversion rate (% ± S.D.) b
10 µl (2 mM)	2	95±3.6	5±1.5
	6	83±2.5	10±3
	24	20±2.5	0
20 µl (4 mM)	2	90±1.7	10±1.5
	6	65±9	30±5.5
	24	10±3.6	2±1
40 µl (8 mM)	2	85±12	1±1
	6	20±3.6	5±2
	24	0	0

^aTreatment solution (Merck): Hydrogen peroxide 30%, cadmium 0.02 ppm, cobalt 0.02 ppm, copper 0.02 ppm, iron 0.1 ppm, nickel 0.02 ppm, lead 0.02 ppm, zinc 0.02 ppm, free Sulfuric acid 40 ppm, chlorine 0.5 ppm, phosphate 5 ppm, sulfate 2 ppm.

^bThree independent experiments were done in triplicates.

Conversion rate: percent of cells that were resistant to 0.5% SDS.

FDA: fluorescein diacetate.

S.D.: Standard deviation.