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. 2010 Feb 16;5(2):e9238. doi: 10.1371/journal.pone.0009238

Figure 5. The 53-kDa marker identified as vimentin.

Figure 5

After purification by chromatography and electrophoresis, the 53-kDa marker was cleaved by proteases. (A) peptide fingerprint after GluC endoproteinase digestion obtained by SELDI-TOF analysis on NP20 ProteinChip Arrays. Peptides with measured molecular masses matching those of the computed GluC endoproteinase proteolytic peptides of vimentin are indicated with an asterisk. (B) GluC endoproteinase peptides of vimentin identified by SELDI-TOF are listed according to their masses. (C) Mapping of the peptides identified by either peptide mass fingerprinting or nanoLC-MS/MS to the vimentin sequence. Sequences highlighted in grey correspond to GluC endoproteinase peptides identified in B. Underlined sequences correspond to 60 trypsin peptides identified by nanoLC-MS/MS. Phosphorylated peptides (37-50 amino acid and 70-78 amino acid peptides) are underlined with dots.