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Flow cytometric analyses of cellular uptake assay. (a) Fluorescence histogram of poly(CBA-DAH)/pDNA transfected primary myoblasts. (b) Fluorescence histogram of poly(CBA-DAH-R)/pDNA transfected primary myblasts. (c) Cell count in R2 gated region, bPEI/pDNA at w/w ratio of 0.6:1 is positive control. Untreated primary myoblasts is the background control for calibration. Results were analyzed using WinMDI software version 2.9.
