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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Cell Immunol. 2009 Dec 5;261(2):134. doi: 10.1016/j.cellimm.2009.11.011

Figure 1. CTX treatment induced an increased level of DCs in fresh BM.

Figure 1

C57BL/6 mice were i.p. treated with PBS (n=3 mice/group) or 4mg/mouse CTX (n=6 mice/group) and BM was harvested 3 days after treatments. BM single cell suspensions were prepared and stained with anti-CD11c, anti-CD11b, anti-Ly6G (Gr-1), and anti-CD86 mAbs. (A) Shows the total cell number of BM cells. (B) Shows the expression levels of CD11c and CD11b in the total BM. Cells are gated on CD11c+CD11b+ and CD11c+CD11b− (R2) populations and on CD11c−CD11b+ population (R3). (C) Shows the expression level of Ly6G in the total BM cells. (D) Shows the expression levels of CD86 on cells gated on R2 (gated on CD11c+CD11b+ + CD11c−CD11b+) and R3 (gated on CD11c−CD11b+). *, Statistically significant (P<0.05).

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