Figure 5. DCs generated from BM from CTX-treated mice culture express simlar levels of different TLRs.

C57BL/6 mice were i.p. treated with PBS (n=3 mice/group) or 4mg/mouse CTX (n=6 mice/group) and BM was harvested 3 days after treatments. BM single cell suspensions were prepared and cultured with 10ng/mL of GM-CSF and IL-4. On day 7 of culture, the non-adherent cells were harvested and processed for the real-time PCR analysis. Results are expressed as normalized mean expression.