Figure 7. DCs generated from BM from CTX-treated mice can prime T cells in vivo.
B6 mice (on Ly5.2 background) were treated with PBS and CTX as described in Figure 1 legend. After 3 days of treatment, BM was harvested and cultured with 10ng/mL of GM-CSF and IL-4. On day 7 of the culture, the non-adherent cells were harvested, counted and activated with 10ng/mL LPS for 24 hours. Cells were washed and pulsed with 5μg/mL of SIINFEKL peptide for 2 hours. Cells were then washed and injected through lateral tail vein into recipient B6 mice (on Ly5.2 background; n=4/group) adoptively transferred 1 day before with 1.5 × 106 cells of naïve OT-1 cells harvested from lymph nodes and spleen of B6 mice on Ly5.1 background. (A) Shows the numbers of effector OT-1 cells in the peripheral blood harvested 7 days after vaccination with DCs. (B) Shows the number of memory OT-1 cells in the peripheral blood harvested 3 days after s.c. revaccination with 100μg/mouse SIINFEKL 40 days after priming. *, Statistically significant (P<0.05).