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. Author manuscript; available in PMC: 2011 Feb 10.
Published in final edited form as: Vaccine. 2009 Dec 8;28(6):1454. doi: 10.1016/j.vaccine.2009.11.073

Figure 2. Systemic and local antibody responses in C3H/HeJ mice after primary and secondary L2R immunization.

Figure 2

Sera and local antibody class and isotype-specific anti-chlamydial responses were assayed 30 and 25 days post 1° and 2 ° L2R immunizations, respectively. Antibody titers were expressed as the highest dilution giving an absorbance of at least 0.3 or 3 times that of the control sample (consistently < 0.1). C. muridarum (MoPn) convalescent sera and vaginal washes tested against MoPn EBs were used as a positive control (data not shown). Ten mice were tested for each immunization group and the symbols denote the response of individual mice. Bars denote the mean antibody titer. Note that mice did not generate a chlamydial specific antibody following clearance of a primary immunization. In contrast, all L2R immunized mice produced a predominant IgG2a antibody response following resolution of the secondary immunization. Chlamydial-specific IgA or IgG antibodies were not detected in the vaginal washes of L2R infected mice following resolution of either the 1° or 2° immunization.