Skip to main content
. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Pulm Pharmacol Ther. 2009 Oct 29;23(2):107. doi: 10.1016/j.pupt.2009.10.009

Figure 1.

Figure 1

The fibrinolytic activity of nebulized pf-mtPA was maintained during storage. Samples were incubated (37°C) with fibrinogen (375 μg/mL) for 5 h after which they were subjected to SDS-PAGE (12.5%). Protein bands were visualized by staining the gel with Coomassie blue (freshly nebulized pf-mtPA + fibrinogen, lane 1: 50μg/mL; lane 2: 100μg/mL; lane 3: 250μg/mL and nebulized pf-mtPA after four weeks of storage at 4°C + fibrinogen, lane 6: 50 μg/mL; lane 7: 100 μg/mL; lane 8: 250 μg/mL). Mouse plasminogen (lane 4), plasmin (lane 5) and molecular weight markers (MWM) were used for protein identification and size determination.