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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Curr Opin Cell Biol. 2010 Jan 12;22(1):57. doi: 10.1016/j.ceb.2009.12.010

Figure 2. Protein architecture and force generation in HeLa and yeast kinetochores.

Figure 2

(a) Proposed models for force generation at the vertebrate kinetochore. These schematics show the two possible arrangements of the Ndc80 complex binding to the MT lattice in HeLa kinetochores. The upper model is deduced from distance measurement data obtained by Wan et al., while the lower model is based on the tilted orientation of the Ndc80 bound to MT lattice observed in vitro. (b) Kinetochore architecture obtained from live budding yeast cells. Of particular interest are the positions of Dam1/DASH complex (green ellipsoids) and Ndc80 complex (orange fibrils). The first version depicts the simplest arrangement representing average distances. Other arrangements are also possible, and the second cartoon depicts a proposed model for persistent kMT attachment through the Ndc80 complex. The Mtw1 complex (blue fibrils) is assumed to attain various orientations with respect to the kinetochore-MT axis and allow the Ndc80 complex (orange fibrils) to reach significant distances downstream from the kMT plus-end.

Color key: NDC80 – ORANGE, KNL-1/Spc105 – RED, Mis12/MTW1 – BLUE, Dam1/DASH – GREEN

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