Table 1.
Distance restraints from solid-state MAS NMR measurements.
| # | Retinal β-Ionone Ring - H5 | Distance (Å) |
||
|---|---|---|---|---|
| Meta II | Rho (PDB ID:1U19) |
|||
| A1 | C6 | 207 Met CE41 | < 4 | 5.35 |
| A2 | C7 | 207 Met CE41 | < 4 | 4.9 |
| A3 | C16 | 207 Met CE41 | < 5 | 3.8 |
| A4 | C17 | 207 Met CE41 | < 5 | 4.5 |
| A5 | C16 | 207 Met C41 | < 5 | 4.9 |
| A6 | C16 | 211 His C41 | < 5 | 4.9 |
| A7 | C16 | 208,212 Phe C41*(ring) | < 5 | 4.7-6.2 |
| A8 | C17 | 208,212 Phe C41*(ring) | < 5 | 4.2-6.1 |
| A9 | C5 | 207 Met CE41 | < 5 | 6.7 |
| A10 | C18 | 207 Met CE41 | < 5 | 7.1 |
| A11 | C5 | 121 Gly CA41 | > 6 | 5.1 |
| A12 | C18 | 121 Gly CA41 | > 6 | 3.7 |
| A13 | C5 | 167 Cys CB41 | > 6 | 8.2 |
| A14 | C6 | 167 Cys CB41 | > 6 | 8.0 |
| A15 | C7 | 167 Cys CB41 | > 6 | 7.8 |
|
Retinal Polyene Chain – Protein | ||||
| B1 | C20 | 114 Gly CA41 | < 6 | 7.2 |
| B2 | C20 | 188 Gly CA41 | > 6 | 6.2 |
| B3 | C12 | 114 Gly CA41 | > 6 | 4.6 |
| B4 | C12 | 188 Gly CA41 | > 6 | 4.3 |
| B5 | C12 | 268 Tyr CZ41 | < 5 | 4.9 |
| B6 | C14 | 268 Tyr CZ41 | < 5 | 6.0 |
| B7 | C20 | 268 Tyr CZ41 | < 5 | 4.2 |
| B8 | C12 | 191 Tyr CZ41 | > 6 | 7.5 |
| B9 | C14 | 191 Tyr CZ41 | > 6 | 9.2 |
| B10 | C20 | 191 Tyr CZ41 | > 6 | 8.0 |
| B11 | C12 | 178 Tyr CZ41 | > 6 | 7.2 |
| B12 | C14 | 178 Tyr CZ41 | > 6 | 9.1 |
| B13 | C20 | 178 Tyr CZ41 | > 6 | 9.7 |
| B14 | C12 | 192 Tyr CZ41 | > 6 | 10.5 |
| B15 | C14 | 192 Tyr CZ41 | > 6 | 11.2 |
| B16 | C20 | 192 Tyr CZ41 | > 6 | 10.8 |
| B17 | C19 | 268 Tyr CZ34 | > 6 | 4.3 |
| B18 | C19 | 191 Tyr CZ34 | > 6 | 4.7 |
| B19 | C19 | 118 Thr C*41 | < 5 | 4.5-6.4 |
| B20 | C14 | 186 Ser CB34 | > 6 | 4.5 |
| B21 | C15 | 186 Ser CB34 | > 6 | 4.1 |
| B22 | C19 | 188 Gly CA34 | > 6 | 4.6 |
| B23 | C9 | 189 Ile C*42 | > 6 | 3.6-6.0 |
| B24 | C12 | 187 Cys C=O42 | > 6 | 4.2 |
| B25 | C20 | 187 Cys C=O42 | > 6 | 6.2 |
| B26 | C12 | 188 Gly C=O42 | > 6 | 5.6 |
| B27 | C20 | 188 Gly C=O42 | > 6 | 7.5 |
|
Retinal - Trp265 | ||||
| C1 | C19 | 265 Trp CE3,CZ3,CH236 | < 5 | 6.0-10.0 |
| C2 | 121 Gly CA | 265 Trp CE3,CZ3,CH236 | > 6 | 3.7-5.9 |
| C3 | C20 | 265 Trp Ring36 | > 6 | 3.9-8.3 |
| C4 | C18 | 265 Trp Ring41 | > 6 | 3.7-5.1 |
| C5 | C5 | 265 Trp Ring41 | > 6 | 3.7-5.3 |
|
Protein-Protein | ||||
| D1 | 167 Cys CB | 211 His CE41 | > 5 & < 6 | 3.9 |
| D2 | 167 Cys CB | 207 Met CE41 | < 5 | 7.8 |
| D3 | 167 Cys CB | 206 Tyr CZ41 | > 5 & < 6 | 5.3 |
| D4 | 207 Met CE | 211 His CE41 | < 5 | 9.7 |
| D5 | 163 Met CE | 211 His CE41 | < 5 | 4.0 |
| D6 | 211 His CE | 206 Tyr CZ41 | < 5 | 4.4 |
| D7 | 288 Met CE | 268 Tyr CZ42 | < 5 | 3.9 |
| D8 | 288 Met CE | 191 Tyr CZ42 | < 5 | 5.2 |
| D9 | 288 Met CE | 192 Tyr CZ42 | > 6 | 5.7 |
| D10 | 86 Met CE | 120 Gly CA36 | < 5 | 4.2 |
| D11 | 114 Gly CA | 178 Tyr CZ42 | > 6 | 4.5 |
| D12 | 296 Lys CE | 44 Met CE41 | > 5 & < 6 | 4.7 |
| D13 | 298 Ser CB | 86 Met CE42 | > 5 & < 6 | 4.8 |
| D14 | 298 Ser CB | 301 Tyr CZ42 | < 4 | 4.9 |
| D15 | 264 Cys CB | 301 Tyr CZ42 | < 5 | 4.8 |
| D16 | 316 Cys CB | 65 His CE142 | > 6 | 3.6 |
| D17 | 160Thr CA/CB | 126/161 Trp (ring) 42 | < 5 | (4.7-8.9)/(4.8-8.7) |
*
All carbons of the amino acid.