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. Author manuscript; available in PMC: 2011 Feb 26.
Published in final edited form as: J Mol Biol. 2009 Dec 21;396(3):602. doi: 10.1016/j.jmb.2009.12.020

Figure 4.

Figure 4

(A) and (B), EPR spectra of the spin-labeled soluble VAMP2 in the truncated complexin Δ 26-SNARE quaternary complex at room temperature. First-derivative mode EPR spectra for the complex in the detergent (A) and in the membrane (B) are shown. The arrowheads indicate the sharp component from the locally displaced VAMP2. The percentage of the sharp component was calculated using the spectral subtraction method (ref. 42). (C), EPR spectra of the spin-labeled full-length and truncated complexin upon binding with the ternary SNARE complex at room temperature. The inset figures show the difference between full-length and truncated complexin involved in the quaternary complex clearly.