Figure 1.

Western blots of deglycosylated EAATs. A. Western blot analysis of EAAT1, EAAT2, and EAAT3 deglycosylated with the enzymes Endoglycosidase H and PNGase F. EndoH and PNGase F lanes indicate enzyme treated samples. EndoH control and PNGase F control lanes were treated identically to the corresponding enzyme treated samples except the enzymes were omitted. Molecular masses of EAAT1 and EAAT2 monomers and multimers were shifted in the PNGase F treated lanes. No shift was detected for EAAT3. B. Western blot analysis of EAAT1 deglycosylated with EndoH and PNGase F in anterior cingulate cortex and dorsolateral prefrontal cortex from a patient with schizophrenia and a comparison subject. Abbreviations: kDa (kilodaltons), excitatory amino acid transporter (EAAT), endoglycosidase H (EndoH), peptide-N⁴-(N-acetyl-beta-glucosaminyl) asparagine amidase F (PNGase F).