Table 5.
In vivo growth of F. tularensis SCHU S4 following aerosol challenge of vaccinated mice.
| Vaccine group | Tissue | Log10 SD bacterial burden on day post-aerosol-challenge: | ||||
|---|---|---|---|---|---|---|
| 2 | 4 | 7 | 10 | 14 | ||
| Naive | lungs | 6.09± 0.11 | 7.71± 0.20 | |||
| LVS | 6.0± 0.17 | 7.52± 0.07 | 8.31± 0.20 | |||
| Δ0918γΔcapB | 5.65± 0.141,2 | 6.37± 0.211,2 | 8.24 ± 0.08 | 8.60 (1/1) | ||
| ΔclpB | 4.79 ± 0.931,2 | 5.94 ± 0.461,2 | 6.27± 0.962,3 | 6.91 ± 0.96 | 3.97± 1.83 | |
| Naive | liver | 4.04± 0.88 | 7.99 ± 0.25 | |||
| LVS | 3.07 ± 0.58 | 5.77 ± 0.131 | 7.94± 0.73 | |||
| Δ0918ΔcapB | 2.68 (3/4) | 4.25± 0.451,2 | 6.23 ± 0.412 | 7.66 (1/1) | ||
| ΔclpB | 1.87 (2/4)1 | 4.26 ± 0.411,2 | 4.14± 0.642,3 | 5.33± 0.70 | 3.70 (3/4) | |
| Naive | spleen | 3.85± 1.0 | 8.26 ± 0.98 | |||
| LVS | 2.13± 0.761 | 5.75 ± 0.171 | 7.54 ± 0.92 | |||
| Δ0918ΔcapB | 2.36 (3/4) | 4.10 ± 0.181,2 | 5.50 ± 0.372 | 6.23 (1/1) | ||
| ΔclpB | 2.43 ± 0.461 | 3.76 ± 0.381,2 | 3.63 ± 0.582,3 | 4.52± 0.82 | 3.41 ± 1.43 | |
| Naive | Blood /mL | 2.82± 0.94 | 7.19 ± 0.03 | |||
| LVS | 3.47 (1/4)1 | 3.22 ± 0.871 | 4.22 (2/4) | |||
| Δ0918ΔcapB | <2.01 | 3.0 (1/4)1 | 3.91± 0.78 | 3.56 (1/1) | ||
| ΔclpB | 2.85 (1/4)1 | 2.0 (1/4)1 | 2.0 (1/4) | 4.38 (2/4) | <2.0 (0/4) | |
Mice (n=4 / group were immunized ID with 105 CFU of one or other attenuated strain of F. tularensis, then challenged 6 weeks later with an aerosol of SCHU S4.
1
burden significantly lower than in naïve mice
2
burden significantly lower than in LVS-immunized mice
3
burden significantly lower than in SCHU S4Δ0918ΔcapB immunized mice