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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Cell Death Differ. 2009 Sep 25;17(3):398–407. doi: 10.1038/cdd.2009.135

Figure 4. p73 is required for vitamin D mediated osteoblastic differentiation.

Figure 4

A) SaOS2 cells transfected with control siRNA or p73 siRNA were harvested and re-plated onto 6 well plates. Next day, cells were treated for 48 hr post vehicle or 10 nM or 100 nM VD3 and total RNA was extracted and transcript levels of p73, VDR, OPN and OCN were determined. Y-axis represents the fold change in transcript levels compared to control siRNA transfected cells with vehicle treatment. Error bars represent standard deviation from the mean. B) SaOS2 cells transfected with control siRNA or p73 siRNA were split and re-plated onto 6 wells. Next day, cells were treated with vehicle or 10 nM VD3 as indicated and at 48 hr post VD3 treatment morphological changes in cells were observed by phase contrast microscopy. C) SaOS2 cells were transfected with control or p73 siRNA and subsequently treated with vehicle or 100 nM VD3 for 24 hrs. Whole cell lysates were then collected and alkaline phosphatase activity was measured. Y-axis represents the relative AP activity normalized to total protein.