Skip to main content
. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Cell Death Differ. 2009 Sep 25;17(3):398–407. doi: 10.1038/cdd.2009.135

Figure 6. DNA damage potentiates vitamin D mediated differentiation via p73.

Figure 6

A) SaOS2 cells were transfected with either control siRNA or p73 siRNA as indicated and treated with 4 μM etoposide or left untreated as indicated. Next day, cells were either cultured in vehicle or 10 nM VD3 for additional 48 hr as indicated. Transcript levels of p73 & VDR were determined. Total protein was extracted and subjected to immunoprecipitation for p73 detection or to standard Western blot analysis for VDR and β-actin. B) Transcript levels of OPN & OCN were determined as in (A) after siRNA, drug and VD3 treatment. Y-axis represents the fold change in transcript levels compared to control siRNA transfected cells treated with vehicle. Error bars represent standard deviation from the mean.