Skip to main content
. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: Toxicol Lett. 2009 Dec 1;192(3):387. doi: 10.1016/j.toxlet.2009.11.016

Fig. 6. Activation of isolated NPC and KC following treatment with rHSP-70.

Fig. 6

NPCs were isolated from naïve female C57Bl/6J mice, cultured in a 96 well plate, and treated with rHSP-70 for 24 hr. Cells cultured in RPMI media alone served as control. Total RNA was extracted and the mRNA expression of inflammatory mediators by NPC was determined by RT-PCR analysis. Densitometric quantification of (A) TNF-α and (B) IL-1β mRNA expression by NPC. *, P < 0.05, compared with cells cultured in media alone (C) KC were purified via F480+ bead selection from NPC isolated from naïve female C57Bl/6J mice, cultured in a 96 well plate, and treated with rHSP-70 in the presence or absence of PMB. mRNA expression of TNF-α, MCP-1 and IL-1β by KC was determined by RT-PCR analysis.