TAMH cells were treated with APAP (0, 1, 10 or 30 mM) for 15 hr. Following treatment, cellular supernatant was collected, concentrated and residual APAP was removed via a desalting column. (A) Immunoblot analysis of HMGB1 release into the cellular supernatant. (B) Quantification of HMGB1 release into the cellular supernatant by densitometry. *, P < 0.05, compared with cells treated with 0 mM APAP.